Determination of Volatile Compounds in Grass and Maize Silages using SPME and GC-MS

Authors

  • Š. Chmelová Department of Biology, Pedagogical Faculty, University of South Bohemia, České Budějovice,
  • J. Tříska Institute of Systemic Biology and Ecology, Academy of Sciences of the Czech Republic, České Budějovice,
  • K. Růžičková Institute of Systemic Biology and Ecology, Academy of Sciences of the Czech Republic, České Budějovice,
  • P. Kalač Department of Applied Chemistry, Faculty of Agriculture, University of South Bohemia, České Budějovice

Abstract

Volatile compounds from grass and maize silages were detected using solid-phase microextraction (SPME), headspace SPME, and GC-MS. Two grass silages were tested untreated, preserved with formic acid and inoculated with Lactobacillus plantarum, or with wilted grass up to 400 g kg-1. Two maize silages were tested untreated, inoculated with L. plantarum or L. buchneri or with a mixture of lactic acid bacteria Microsil. The silages were fermented and stored for 4 months. Volatiles were isolated by headspace SPME. Using this method, 21 volatile compounds, fatty acid esters, terpenes and phenolics were detected. The contents of 4-ethylphenol and 2-methoxy-4-vinylphenol were determined.

Published

2009-01-15

How to Cite

Chmelová, Š., Tříska, J., Růžičková, K., & Kalač, P. (2009). Determination of Volatile Compounds in Grass and Maize Silages using SPME and GC-MS. Chemické Listy, 102(12). Retrieved from http://blog.chemicke-listy.cz/ojs3/index.php/chemicke-listy/article/view/1588

Issue

Section

Articles