Application of the LAMP Method for Detection of Undesirable Bacteria in Whey

Abstract

The LAMP method (Loop-Mediated Isothermal Amplification) is a molecular biological method for the amplification of specific DNA and RNA sequences suitable for the detection of various pathogenic, semi-pathogenic and technological undesirable microorganisms. The aim of the work is using the LAMP method for the option verification of detection of health and technologically undesirable bacteria in liquid sweet whey under laboratory conditions. Experiments were carried out with 12 bacterial strains, including three representatives of Gram-positive bacteria (Enterococcus spp., Streptococcus sp.) and 9 representatives of Gram-negative bacteria (E. coli, Kl. pneumoniae, Ps. aeruginosa). The Eazyplex^® BloodScreen GP/GN Assay kits (AmplexDiagnostic GmbH, Germany), designed for Gram-positive (GP) and Gram-negative (GN) bacteria and commonly used in clinical laboratory practice, were used for the LAMP experiments. Based on the obtained results it was concluded that the LAMP method was suitable for the detection of tested bacteria at the level of the species in liquid sweet whey. The results of this work can be used in the application of the LAMP method in dairy laboratory practice, in the targeted assurance of health safety and quality of food raw materials and products, especially liquids.