LC-MS - an Objective Diagnostic Method of Amanita Intoxications

Authors

  • B. Merová Institute of Forensic Medicine, Faculty of Medicine, Palacky University, Olomouc
  • M. Staňková Institute of Forensic Medicine, Faculty of Medicine, Palacky University, Olomouc | Institute of Forensic Medicine, University Hospital Ostrava, Ostrava
  • J. Stříbrný Institute of Forensic Medicine, Central Military Hospital, Prague
  • P. Ondra Institute of Forensic Medicine, Faculty of Medicine, Palacky University, Olomouc

Keywords:

amanita, LC-MS, muscarine, amatoxins, phalotoxins, intoxication

Abstract

A LC-MS method for determination of muscarine and amatoxins in urine and blood serum was developed and validated. The analysis was carried out using an LC/MS quadrupole device equipped with electrospray. The separation of muscarine, α-amanitin, β-amanitin, phalloidin and phallacidin was performed on analytical columns in the gradient mode. The applicability of the method has been demonstrated by analyzing clinical samples.

Downloads

Published

2012-09-15

How to Cite

Merová, B., Staňková, . M., Stříbrný, J., & Ondra, P. (2012). LC-MS - an Objective Diagnostic Method of Amanita Intoxications. Chemické Listy, 106(9), 831–835. Retrieved from http://blog.chemicke-listy.cz/ojs3/index.php/chemicke-listy/article/view/4290

Issue

Vol. 106 No. 9 (2012)

Section

Articles