Development of Solid-Phase Extraction and HPLC/MS Methods for Deoxynivalenol Determination in Barley and Malt

Authors

  • Alena Ježková Department of Food Technology, Faculty of Agronomy, Mendel University of Agriculture and Forestry, Brno
  • Jana Žďárová Karasová Department of Toxicology, Faculty of Military Health Sciences, University of Defence, Hradec Králové
  • Vlastimil Dohnal Department of Food Technology, Faculty of Agronomy, Mendel University of Agriculture and Forestry, Brno | Department of Toxicology, Faculty of Military Health Sciences, University of Defence, Hradec Králové | Department of Chemistry, Faculty of Science, J.E. Purkyně University, Ústí nad Labem
  • Ivana Polišenská Agrotest Fyto Ltd, Kroměříž

Keywords:

mycotoxin, deoxynivalenol, HPLC-MS, barley, malt

Abstract

A method for the determination of deoxynivalenol (DON) in cereals has been developed and tested. The method is based on solid phase extraction in a column and subsequent separation by HPLC-MS. DON was detected with a MS detector in positive mode. The method is inexpensive, fast and highly sensitive. The method was used for the determination of deoxynivalenol in barley and malt, with the detection limit 25-30 μg kg-1. Changes in the DON content after malting were followed using the method.

Downloads

Published

2009-08-15

How to Cite

Ježková, A., Žďárová Karasová, J., Dohnal, V. ., & Polišenská, I. (2009). Development of Solid-Phase Extraction and HPLC/MS Methods for Deoxynivalenol Determination in Barley and Malt. Chemické Listy, 103(8), 679–683. Retrieved from http://blog.chemicke-listy.cz/ojs3/index.php/chemicke-listy/article/view/4365

Issue

Vol. 103 No. 8 (2009)

Section

Articles