Determination of 1-Hydroxypyrene Using High-Performance Liquid Chromatography with Electrochemical Detection

Authors

  • Jiří Barek Department of Analytical Chemistry, Charles University, Prague
  • Vladimír Bencko Department of Hygiene and Epidemiology, Charles University, Prague
  • Josef Cvačka Department of Analytical Chemistry, Charles University, Prague
  • Viktor Mejstřík Research Institute for Organic Syntheses, Pardubice
  • Alena Slámová Department of Hygiene and Epidemiology, Charles University, Prague
  • Ivana Švagrová Department of Analytical Chemistry, Charles University, Prague
  • Jiří Zima Department of Analytical Chemistry, Charles University, Prague

Abstract

Optimum conditions are described for HPLC determination of 1-hydroxypyrene with electrochemical detection (ED) giving the detection limit – 1.10–8 mol L–1. This detection limit was compared with that for UV spectrophotometric detection (–1.10–7 mol L–1) and fluorimetric detection (~ 5.10–10 mol L–1). It was demonstrated that HPLC-ED can be used for the determination of 1-hydroxypyrene in urine down to 1.10–7 mol L–1 and thus for biological monitoring of human exposure to polycyclic aromatic hydrocarbons.

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Published

1997-10-15

How to Cite

Barek, J., Bencko, V., Cvačka, J., Mejstřík, V., Slámová, A., Švagrová, I., & Zima, J. (1997). Determination of 1-Hydroxypyrene Using High-Performance Liquid Chromatography with Electrochemical Detection. Chemické Listy, 91(10), 871–876. Retrieved from http://blog.chemicke-listy.cz/ojs3/index.php/chemicke-listy/article/view/4393

Issue

Vol. 91 No. 10 (1997)

Section

Articles