Development and Application of Electromigration Methods at the Institute of Organic Chemistry and Biochemistry of the Czech Academy of Sciences
The article is additionally devoted to the 70ᵗʰ anniversary of the founding of the Institute of Organic Chemistry and Biochemistry of the CAS in Prague and currently to the 100ᵗʰ anniversary of the founding of the Department of Analytical Chemistry of the Faculty of Science of Charles University in Prague and to the significant life anniversary of Professor Jiří Barek
DOI:
https://doi.org/10.54779/chl20240579Keywords:
capillary electrophoresis, isotachophoresis, isoelectric focusing, affinity electrophoresis, electrokinetic chromatography, electrochromatographyAbstract
This article summarizes the development and application of electromigration methods at the Institute of Organic Chemistry and Biochemistry of the Czech Academy of Sciences in Prague (IOCB Prague) since the founding of the Institute up to the present time. The development of instrumentation, methodology and applications of the following methods is described: (i) non-cooled and cooled paper electrophoresis and its application for both analytical and semipreparative separation of amino acids and peptides for determination of primary structure of proteins; (ii) continuous flow-through electrophoresis in a free solution (FFE) applied for purification of biologically active peptides, especially peptide hormones, with the preparative capacity of 50–100 mg h–1; (iii) capillary isotachophoresis (CITP) used for the quality control of synthetic or isolated biological active peptides and their counterions; (iv) high performance capillary electromigration methods (HPCE) including zone electrophoresis, isotachophoresis, isoelectric focusing, affinity electrophoresis, electrokinetic chromatography, and electrochromatography, and their application for separation, analysis and physicochemical and biochemical characterization of a wide spectrum of (bio)molecules isolated, (bio)synthesized and investigated at the IOCB, e.g. amino acids, peptides, proteins, nucleosides, nucleotides, fragments of nucleic bases, steroids, catecholamines, and various functional organic molecules, such as azahelicenes, helquats and diquats.
