Overproduction and Characterization of UvrA Protein Encoded by Plasmid from Acetobacter estunensis GP2

Authors

  • P. Grones Department of Molecular Biology, Comenius University, Bratislava
  • S. Mináriková-Vávrová Department of Molecular Biology, Comenius University, Bratislava
  • M. Babič Department of Molecular Biology, Comenius University, Bratislava
  • Z. Odnogová Department of Molecular Biology, Comenius University, Bratislava
  • J. Grones Department of Molecular Biology, Comenius University, Bratislava

Keywords:

bacterial plasmid, uvrA gene, exinuclease A, Acetobacter

Abstract

Plasmid pGP2 from Acetobacter estunensis GP2 encodes 1 296 bp uvrA gene for 432 aa reparation protein exinuclease A. Bioinformatic analysis of the protein confirmed 13 α-helixes and 14 β-structures, two domains of ABC transporters, one GTPase domain and a 21-amino-acid large trans-membrane domain. The protein has 98–88 % identities with some bacterial genome-encoded proteins. uvrA gene amplified by PCR was cloned in pET28a- expression vector and purified by HPLC affinity chromato­graphy. The purified protein (47.17 kDa) shows the ATPase activity ten times lower than the Rep34 protein (isoletectric point 6.8) from plasmid pGP2.

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Published

2012-08-15

How to Cite

Grones, P., Mináriková-Vávrová, S., Babič, M., Odnogová, Z., & Grones, J. (2012). Overproduction and Characterization of UvrA Protein Encoded by Plasmid from Acetobacter estunensis GP2. Chemické Listy, 106(8), 777–782. Retrieved from http://blog.chemicke-listy.cz/ojs3/index.php/chemicke-listy/article/view/876

Issue

Vol. 106 No. 8 (2012)

Section

Articles