The Use of Nonspecific Peptides for Proteomic Identification of Low-Molecular-Weight Proteins from Barley by MALDI-TOF Mass Spectrometry

Authors

  • Pavel Řehulka Institute of Analytical Chemistry, Academy of Sciences of the Czech Republic, Brno, Czech Republic
  • Gunter Allmaier ienna University of Technology, Vienna, Austria
  • Josef Chmelík Institute of Analytical Chemistry, Academy of Sciences of the Czech Republic, Brno, Czech Republic | Research Centre for Study of Extractives from Barley and Hop, Institute of Analytical Chemistry, Academy of Sciences of the Czech Republic, Brno, Czech Republic

Keywords:

non-specific peptides, proteins, proteomics, barley, MALDI-TOF/TOF

Abstract

Nonspecific peptides were analyzed for proteomic identification of low-molecular-weight barley proteins either by MALDI-TOF mass spectrometry involving post-source decay analysis or using a MALDI-TOF/TOF instrument. Identification of low-molecular-weight barley proteins was successfully accomplished by MS/MS analysis of both tryptic and nonspecific peptides in the cases where peptide mass fingerprinting failed. Utilization of nonspecific peptides also increased protein sequence coverage in database searching.

Downloads

Published

2006-12-15

How to Cite

Řehulka, P., Allmaier, G., & Chmelík, J. (2006). The Use of Nonspecific Peptides for Proteomic Identification of Low-Molecular-Weight Proteins from Barley by MALDI-TOF Mass Spectrometry. Chemické Listy, 100(12), 1111–1115. Retrieved from http://blog.chemicke-listy.cz/ojs3/index.php/chemicke-listy/article/view/4379

Issue

Vol. 100 No. 12 (2006)

Section

Articles